Background: SR-971, a functional food used for its anti-cancer, anti-aging, and antioxidanteffects, was assessed for its genotoxic potential in in vitro and in vivo genotoxicity assays. These included a bacterial reverse mutation assay (Ames test) and an in vivo rodent hematopoietic cell micronucleus assay.
Materials and methods: Negative and positive control cultures and animals were used in all assays. In the Ames test, varying concentrations of SR-971 were used to assess the mutagenicity in five strains of Salmonella typhimurium (TA1537, TA98, TA100, TA102, and TA1535) in the presence and absence of the S9 rat liver metabolic activation system. In the micronucleus assay, mice were randomly divided into five groups: low dose (0.5 g/kg bw), middle dose (1 g/kg bw), and high dose (1.67 g/kg bw) of SR-971, positive control (cyclophosphamide, 200 mg/kg bw), and negative control (ddH2O). Blood was collected at 24, 48, and 72 h post administration.
Results: SR-971 showed no mutagenic effects at the highest dose (5 mg/plate) in the presence or absence of metabolic enzymes in the Ames test. In the micronucleus assay, SR-971 had no effect on the number of micronuclei at any dose or sampling time.
Conclusion: Administration of SR-971 as a functional food does not pose a genotoxic risk to humans and target animal species.

Head, shoulder, and neck pain has become a common health problem around the world. It is a typical joint and muscle pain and is more likely to occur in middle-aged, elderly, and sedentary office workers among sub-healthy people. Nowadays, relief to stiff shoulders and stiff neck pain is commonly done by acupuncture, Chinese herbal medicine, hot compress, cupping, and chiropractic massage. The related research literature on the application of traditional Chinese chiropractic manipulation massage or essential oil therapy to improve shoulder and neck pain is relatively rare; therefore, the objective of this study is to investigate the effects of chiropractic massage and essential oils on improving shoulder and neck pain. The study mainly discusses the effects of traditional Chinese chiropractic massage and essential oil therapies on the improvement of shoulder and neck pain. An experimental case study was conducted. The data were collected and used to compare the efficacy of traditional chiropractic manipulation massage versus essential oil therapy. Based on the data collected from individual case studies, to compare their differences before and after the measured values of heart rate variability (HRV), parasympathetic nerve, etc., also including the qualitative research of individual cases to obtain more detailed concepts, this way analysts can conclude whether essential oil massage and chiropractic manipulation are effective in relieving shoulder and neck pain.